한국콩연구회

분자육종기술을 이용한 알러젠 프리 고품질 기능성 콩 계통 육성 및 산업화

Industrialization of allergen free soybean line and development of high-value traits soybean lines using molecular genetics

주관연구기관 : 건국대학교

연구책임자 : 정우석

발행년월 : 2011-02

주관부처 : 농촌진흥청

원문

http://www.ndsl.kr/ndsl/commons/util/ndslOriginalView.do?dbt=TRKO&cn=TRKO201100016011&rn=&url=&pageCode=PG18

초록

The research entitled 'Developing allergen free elite soybean lines and commercialization using molecular breeding technology' was conducted in five different research teams. Ultimate goals of the research were launching new soybean products made with previously developed soybean cultivars and developing gene specific molecular markers for breeding, particularly related to lipid biosynthesis, developing P34 allergen free elite lines and its gene specific molecular maker and cloning and function characterization of biotic and abiotic stress resistance genes and developing their molecular markers. We developed two soybean products with Kti free soybean line and lipoxygenase activity suppressed line. Those two products were launched to market in Korea and abroad. Also, we screened overall 450 lines cumulating through F2 to F4 generation and selected high oleic, low linoleic and linoleic fatty acid lines. Additionally we analyzed isoflavone contents of those lines. To develop P34 allergen free lines which have acceptible agicultural characteristics, we crossed small seed cultivars with PI567476 and currently we have F5 plants. Also, we have crossed and created various soy bean lines which have particular traits such as high oleic, low linolenic etc. We have characterized P34 allergen. We analyzed P34 gene coding and promoter region compared with normal counterpart and expressed P34 gene in microorganism and made polyclonal antibodies. In addition we analyzed seed proteins and isolated P34 protein spot and measured molecular mass and checked the molecular characteristics of P34 protein through MALDI -TOF analysis to identify polymorphic characters along with P34 gene expression pattern in soy bean plant. We developed P34 gene specific molecular markers. For the biotic and abiotic stress resistance marker and analyze stress related gene expression and function, we used soy bean and Arabidopsis model system. We have checked various genes which response to low temperature, physical lesion and low oxygen concentration in air. To figure out related gene's expression and function we used transgenic Arabidopsis and have analyzed various knock out or over-expression transgenic mutants. Through this research we reported three academic papers and we made 19 patent applications and among them patents were granted on 6 applications. Also, we reported 9 cloned and characterized genes and 1 transgenic plants. We registered 2 soybean cultivars.

가. 확보중인 소화억제단백질 (쿠니츠트립신인히비터) 결핍 소립 검정콩 계통의 농가 재배확대 및 수요

확대를 위한 제품개발

나. 콩 품질 및 기능성관련 분자마커 개발

다. 고품질 소립 검정콩 계통의 육성

라. P34 단백질 프리 고 품질 콩 계통 육종

마. P34 단백질의 유전 및 타 형질과의 연관 분석

바. P34 유전자의 분자유전학적 특성 구명 및 유전자 특이적 분자마커 개발

사. 선발된 우수 계통의 품종화를 위한 농업적 형질 조사

아. 기후변화에 대비한 콩 내재해성 형질 연구

자. 콩에서 저온, 상처, 및 저산소 스트레스에 의해 특이적으로 유도되는 유용 유전자 W23, W143, LT248 그리고 전사조절인자의 기능분석

차. 저온 및 습해에 강한 콩 유전자원 중간 모본 개발

카. 내재해 관련 유용 유전자, SLTI66, SLTI629, MLT92과 전사조절인자의 스트레스 저항성 분석 및 조직 특이 프로모터 개발