한국콩연구회

콩 내습·내한발성 관련 분자표지 및 저항성 자원 개발

Development of research molecular marker and resistance sources for soybean flooding and drought

주관연구기관 : 국립식량과학원

연구책임자 : 최만수

발행년월 : 2016-02

주관부처 : 농촌진흥청

원문

http://www.ndsl.kr/ndsl/search/detail/report/reportSearchResultDetail.do?cn=TRKO201600003120

초록

Soybean [Glycine max. (L.) Merr] is one of the most important legumes in the world. However, soybean varieties are sensitive to flooding stress and their seed yields are substantially reduced in response to the flooding stress. 192 soybean germplasm collection was screened to identify flooding tolerant germplasm at an early vegetative growth stage (V1). Soybean plants at V1 stage were waterlogged for 4 to 10 days. To evaluate flooding tolerance, survival rate were investigated as a time dependent manner. Jangbaegkong, Danbaegkong, Sowonkongkong, Socheong2 and Suwon269 showed flooding tolerance, while Shillog, T201, T181, NTS1116 and HP-963 showed flooding sensitivity. We also investigated effects of flooding stress on soybean morphology. The adventitious root development was greatly increased in flooding tolerant plants compared to it in flooding sensitive plants. In addition, root length and root number were analyzed. The significant reduction of root length and root number was observed in flooding sensitive plants. Thus, these results indicate that the morphological changes in roots are important for acclimation to flooding stress. Taken together, the relationship between the morphological changes in the roots and flooding tolerance may be useful in selecting a flooding tolerant soybean germplasm.

Drought is one of the major constrains for soybean production worldwide and genetic improvement for drought tolerance is a effective approach to limit soybean yield loss under drought conditions. In order to identify drought tolerant soybean genotypes, 98 sybean germplasm collection was screened at an early growth stage. Based on leaf relative water content (RWC) and wilting scores under drought at growth chamber, 12 genotypes were selected as drought tolerant candidates. To confirm whether 12 candidates show drought tolerance in green house, leaf wilting scores and drought index were investigated in comparison with two check cultivars, PI416937(tolerant) and Seonyu(sensitive). Among the tested genotypes, Songhag, Ilpumgeomjeong2 and Seonpung showed significantly higher drought index and lower wilting score than the drought tolerant check PI416937. To elucidate the effects of drought stress on the change of physiological properties in soybean seed, nutrient composition and antioxidant activity were determined. Total fatty acid and antioxidant activity were significantly increased in Songhag, Ilpumgeomjeong2 and Seonpung by drought stress, while total protein was slightly decreased. The change of physiological properties in the drought tolerant genotypes was similar with that in PI416937. The results demonstrate that the physiological changes could contribute to acclimation of soybean genotypes in response to drought condition. In this study, we identified some drought tolerant genotypes. Thus, the use of the improved drought tolerant genotypes in traditional breeding program will facilitate the development of elite soybean cultivar with higher yield potential and stability in drought environments.

연구개발 결과

<제1세부 과제 : 콩 주요 내습성 유전자원 선발 및 분자표지 개발 >

○ 생육초기 습해 저항성과 감수성 유전자원 선발

- 습해 저항성 유전자원: 장백, 팔달, 단백, 소원, 소청2호, 수원269호

- 습해 감수성 유전자원: 실록, 밀양217호, T181, T201, NTS1116, HP-963

○ 분자생물학적 방법으로 습해관련 유전자 발현 분석

- 습해 감수성과 저항성을 활용한 Methyl-seq 결과 분석

- 습해 감수성과 저항성을 활용 RNA-seq으로 발현차이 분석

- 습해 감수성과 저항성을 활용한 단백질 발현 분석

○ 생산력검정 계통에 대한 습해저항성 검정

- 숩해 저항성 계통 선발: 8계통 선발(장류용 2, 특수용 1, 나물용 5)

<제2세부 과제 : 콩 내한발성 유전자원 선발 및 분자표지 개발>

○ 한발 저항성 콩 유전자원 선발

- 개화시기의 한발 저항성 콩 자원: 신화, 일품검정, 대흑

- 생육초기의 한발 저항성 콩 자원: 송학, 대망2호, 장일, 선풍

○ 분자생물학적 방법으로 한발관련 유전자 발현 분석

- 한발처리에 의해 감소하는 유전자: GmDREBb와 GmWRKY57

- 한발처리에 의해 증가하는 유전자: GmDREBa, GmERF3, GmGT-2A, GmGT-2B

○ 한발 저항성 품종 개발을 위한 집단 작성

- 선풍, 송학, 일품검정2호를 모본, PI416937을 부본으로 인공 교배하여 집단 작성

- 개화시기의 한발 저항성 유전분석 집단: 모본(PI416937), 부본(진품, 해품, 전상, 우람)

- 생육초기의 한발 저항성 유전분석 집단: 모본(선풍, 송학, 일품검정2호), 부본(PI416937)