한국콩연구회

우리나라 콩 논 재배를 위한 콩 씨스트 선충 저항성 연구

Studies on improvement of resistance to soybean cyst nematode for the safe cultivation of soybean in paddy field in Korea

주관연구기관 : 부산대학교 산학협력단

연구책임자 : 최인수

발행년월 : 2014-02

주관부처 : 농촌진흥청

원문

http://www.ndsl.kr/ndsl/commons/util/ndslOriginalView.do?dbt=TRKO&cn=TRKO201400011369&rn=&url=&pageCode=PG18

초록

The study entitled "studies on improvement of resistance to soybean cyst nematode for the safe cultivation of soybean in paddy field in Korea" has been conducted for the purposes of 1) to reduce yield loss of soybean due to soybean cyst nematodes and 2) to establish sustainable soybean cultivation system in the paddy field. This study is composed of 3 sub-studies; 1) studies on distribution of soybean cyst nematode in the soybean field and selection of soybean cultivars resistant to soybean cyst nematode, 2) Genetic and molecular biological studies for the regeneration of soybean cultivars resistant to soybean cyst nematode, and 3) study of resistance to soybean cyst nematode for the sustainable soybean cultivation and production. In the study of distribution of soybean cyst nematode and selection of resistant cultivars, we surveyed HG type for 13 soybean cyst nematode population. Soybean cyst nematode was most prevalent in the soybean field. Six HG types( 0, 2, 5, 2.5, 1.2.7, and 2.5.7) were identified. The frequency of HG type 2.5 was 30.8%, and that of HG type 2.5.7 was 23.0%. From the bioassay of resistance to soybean cyst nematode for the soybean varieties in Korea, we found out that there was not any soybean cultivar resistant to soybean cyst nematode, which suggest that it's crucial and urgent to develop soybean cultivar resistant to soybean cyst nematode. In the genetic and molecular biological studies for the regeneration of soybean cultivars resistant to soybean cyst nematode, we tested QTLs, genes, and proteins, which previously known to resistant to soybean cyst nematode. From QTLs analyses, we couldn't find any results to apply for utilization to soybean breeding. From the analyses of genes(rhg1, rhg2, rhg3, and Rhg4) resistant to soybean cyst nematode, we revealed that there are SNP variations between resistant and susceptible cultivars. From the subsequent analyses, we identified the variations for genes and protein structures for rhg1, rhg2, rhg3, and Rhg4 genes. From the three dimensional analyses for both rhg1 and rhg2 genes, we explained the mechanism for resistance and susceptibility for resistant to soybean cyst nematode. From RAPD analysis, we suggest the possibility of identification a new gene resistant to soybean cyst nematode for the gene using primer OPY03 that contain serine-threonine kinase domain, which has been reported that this gene might related to resistance to soybean cyst nematode(Hauge et al., 2001). Utilization of PI90763, PI437654, and PI89772 could accelerate regeneration of soybean cultivars resistant to soybean cyst nematode. In the study to establish soybean cultivation system in the paddy field, we identified that cultivation of both rape and perilla for 2 years showed significant reduction of soybean cyst nematode in the soybean field.

연구개발 결과

○ 국내 콩 기생성선충 종류 및 분포 조사

○ 국내 콩 씨스트선충의 HG type 조사

○ 국내 콩 장려품종의 콩 씨스트선충 HG type 2.5에 대한 저항성

○ 콩 씨스트선충 저항성 국외 유전자원 수집 및 활용

○ 콩 씨스트 선충 저항성 QTLs 분석

○ 콩 씨스트선충 저항성 유전자 특성 조사

○ 콩 씨스트선충 저항성 유전자 구조 분석

○ 콩 씨스트선충 저항성 단백질 구조 분석

○ RAPD 분석을 통한 콩 씨스트 선충 저항성 유전자 탐색

○ 유기물 처리에 의한 콩 씨스트 선충의 방제