한국콩연구회

두(콩)류자원으로부터 생물반응기를 이용한 태좌조직배양기술의 산업적 응용

Industrial utilization for phytoplacenta tissue culture technology using bioreactors from leguminous grain crops resources

사업명 : 생명산업기술개발

주관연구기관 : (주)바이오에프디엔씨

연구책임자 : 모상현

발행년월 : 2013-10

주관부처 : 농림축산식품부

원문

http://www.ndsl.kr/ndsl/commons/util/ndslOriginalView.do?dbt=TRKO&cn=TRKO201400030175&rn=&url=&pageCode=PG18

초록

Chemically diverse natural products are derived from plants. The production of plant secondary metabolites by means of large scale culture of plant cell in bioreactors offers an attractive option for producing many phytochemicals. The soybean(Glycine max) is a species of legume native to East Asia, widely grown for its edible bean which has numerous uses. The beans contain a variety of biologically active secondary metabolites such as phytic acid, alpha-linolenic acid and isoflavones. Moreover, Soybean extracts are being used as cosmetic ingredients such as antioxidants, thickening agents and moisturizing agents. In the present study, soybean placenta was sterilized cultured in bioreactors and extraction was carried out in 50% ethanol. Treatment with soybean placenta culture extract enhanced the expression of collagen synthetic markers and procollagen type I C-peptide(PIP). Soybean placenta cells by large-scale culture may provide a cost-effective and environmentally friendly platform for sustainable production of a variety of important plant natural products for cosmetic application. Therefore, this study suggests that soybeans(Glycine max) placenta culture extract can be developed as topical application of natural anti-aging cosmeceuticals by enhancement of collagen synthesis. On the 2nd project: Production of biomass and bioactive compounds by placenta cell suspension cultures of Glycine max(L.) in a liquid-phase airlift balloon-type bioreactor.

To improve biomass and production of bioactive compounds such as daidzein, phenolics, and flavonoids by placenta cell cultures of Glycine Max, the effects of aeration rate, inoculum density were investigated using a 20 L balloon-type bubble bioreactor. Overall, an aeration rate of 0.05 vvm, 15 g/L-1 inoculum density, 1% sucrose concentration were optimal for enhancing accumulation of Glycine max placenta cell dry biomass (4.38 g/L-1), phenolics (54.81 mg/L-1 DW), and flavonoids (49.27 mg/L-1 DW).

연구개발 결과

식물조직배양기술을 적용하여 두(콩)류 자원으로부터 태좌조직부위를 순수 분리·배양하여 연중 균일한 생리활성물질을 생산할 수 있는 최적화 대량생산 공정을 확립하였다. 또한, 생리활성물질의 분석 표준화, 유효성분 분리·동정 및 유효성 평가를 통하여 화장품 기능성 원료로의 신소재 개발을 체계적으로 확립하였다. 대용량 생물반응기에서 생산된 대두태좌조직배양체로부터 추출한 두(콩)류 자원 유래 고부가성 물질은 자연 상태에서 재배한 식물체에서 추출 가능한 유효성분 함량보다 현저히 높았으며, 유효성분 분리·정제 및 분석법을 확립하여 그 효율성을 증가시켰다. 화장품 원료로서의 유효성 평가를 통하여 안티에이징 효과가 뛰어남을 확인하였고, 인체첩포실험을 비롯한 임상실험을 통하여 안정성 및 기능성을 확인하였다. 마지막으로, 대두태좌조직배양추출물을 함유한 화장품 시제품(하이드로겔마스크팩)을 제작하여 우수한 제형으로 개발하였다.