한국콩연구회

고 Isoflavone 콩 계통 육성

Improvement of Soybean Line with High Isoflavone Content

주관연구기관 : 경상대학교

연구책임자 : 정종일

발행년월 : 2005-04

원문 : http://www.ndsl.kr/ndsl/search/detail/report/reportSearchResultDetail.do?cn=TRKO201100015810

초록

Soybean is widely used as a protein source both for humans and domesticated animals. Soybean contains several biologically active components (e.g.isoflavones, saponins, phytic acids, phytosterols, phenolic acids,). Isoflavones are found in soybeans in large amounts. The beneficial effects of isoflavones are reduction on risk of several types of cancer, of cardiovascular disease, of osteoporosis, and attenuation of menopause symptoms. Isoflavone content and composition of soybean seeds is very different according to the morphological part of the seed and genotypes and the environment conditions during seed development. Isoflavones are a group of secondary metabolites produced in plants. They are synthesized as part of the phenylpropanoid pathway. Therefore, breeding of high isoflavone soybean line by classical and molecular breeding techniques and cultivation methods for high isoflavone content are needed.

The present study aims to improve new soybean lines with high isoflavone content and to investigate cultivation method of increasing isoflavone content. Also, purpose of this research is to identify various genetic markers that are linked to loci conditioning variation in isoflavone production and to investigate the structure and expression of the IFS genes in various soybean cultivars.

1. 유전자원의 수집, 명가, 모본의 선정, 집단의 창성, 마커 개발을 위한 집단의 전개

2. 콩 종실 아이소플라본 분석방법 및 체계 확립

3. 고 isoflavone 함량 계통 선발

4. 고 아이소플라본 계통의 농업적 형질 펑가

5. 품종별 IFS 유전자 분리 빛 구조분석

6. IFS 유전자 발현 분석 빛 유전자 프로모터 확보

7. IFS 유전자 발현 조절에 대한 환경요인의 효과 분석

8. 콩의 종실 (sink) 크기별로 아이소플라본과 사포닌 함량 흑은 성분 수량이 최대화 되는

수확기 구명

9. 재배시기 및 지역별 isof1avone 함량 변이 구명

10. 고 아이소플라본 선발 계통 재배시험 및 형질 평가